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CELLSERV KITS AND POWERPOINTS

The Foundation for the Advanced Education in the Sciences
at the National Institutes of Health

Offers innovative, inexpensive self contained kits and PowerPoint presentations for hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

Act today, to enliven your laboratory program by incorporating a CellServ kit and/or a PowerPoint Presentation. Invite the specialist into your classroom and forge a strong teaching alliance by utilizing our resources.

CellServ responds to the pressing need of forward-looking instructors who want to incorporate exciting, novel, cell-culture based exercises into their laboratory programs. Each topic addressed by a CellServ kit, which serves 30 students, provides instructors with an opportunity to expand on the importance of the biological principles associated with the topic at hand. CellServ offers cost-effective materials and instructions that:

  • familiarize students with the underlying principles of cell culture technology and its application to diverse areas of research,
  • permit students to participate in a uniquely different and important technology,
  • allow students to sharpen their observation and quantitative microscopy skills and
  • reduce instructor preparation time.

CellServ also offers an extensive line of PowerPoint Presentations on varied core biotechnology topics to enhance the learning experience of students without increasing the burden on teachers.

CellServ has been in existence since 1989 and has served over 450,000 student-lab units nationwide. It has been adopted by several school districts and is recommended in several published manuals. Attesting to the value of the instructional material is the repeated and expanding use of the service.

Kits

Note to Customers: All CellServ products are shipped out on Mondays. When ordering Kits 4 and 5, please understand that these kits are perishable and time sensitive! Due to the perishable nature of the cells, kit #4 and Kit #5 must be shipped by FedEx 2nd Day or FedEx Overnight and are good for only 2-4 weeks when stored at  0 to -20 Celsius.

For Assistance Call (301) 496-7977 

CSK1 Kit 1: Visualization of Normal and Tumor Cells $65.00
CSK2 Kit 2: Observation of Heterokaryons Through Cell Fusion $65.00
CSK3 Kit 3: Effects of Cytotoxins on Cells $65.00
CSK4

Kit 4: Preparation of Human Chromosome Spreads

(Perishable:  Kit#4 only good for 2-4 weeks when stored at 0 to -20 Celsius)

$65.00
CSK5

Kit 5: Mouse Chromosome Studies

(Perishable: Kit#5 only good for 2-4 weeks when stored at 0 to -20 Celsius)

$65.00
CSK6 Kit 6: Chromosome Banding (includes CSPP8) $99.00

Power Point Presentations
CSPP1 PowerPoint: Normal and Cancer Cells In Vitro $39.00
CSPP2 PowerPoint: Polymerase Chain Reaction (PCR) $39.00
CSPP3 PowerPoint: Principles of Gel Electrophoresis $39.00
CSPP4 PowerPoint: Karyotyping $39.00
CSPP5 PowerPoint: Cell Fusion $39.00
CSPP6 PowerPoint: Toxicology and Toxicity Testing $39.00
CSPP7 PowerPoint: Immunology $39.00
CSPP8 PowerPoint: Chromosome Banding $39.00
CSPP9 PowerPoint: Immunocytochemistry $39.00

Each 35-45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35-40 minute class period.

Kit Descriptions

CSK1 - Visualization of Normal and Tumor Cells

Kit 1: Visualization of Normal & Transformed Cells

Using this kit, each student will be led through a step by step procedure which involves staining, mounting, and observation of normal and transformed (tumor) cells. Each student will observe the morphological differences, as well as the differing growth patterns of these cell types when grown in culture.

The manual provided with this kit describes how the cells are grown in culture, what factors influence the transformation of cells, and the differences in growth behavior between normal and tumor cells in culture. Photographs of these cell types are used to guide the students through the observation steps

L929 transformed mouse cells
Suitable for introductory biology, cytology, cell culture, oncology, and developmental biology courses.

$65.00 Each Kit includes:

  • Enough materials for 30 students working individually.
  • Coverslips with IMR-90, a normal, diploid human cell line which shows the typical fibroblastic morphology and oriented growth pattern
  • Coverslips with L929, a transformed mouse cell which shows cells of varying morphology and giant cells.
  • STAIN #1 and STAIN #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Monocular or binocular microscope (10 - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

30-45 minutes, including casual observations.

These are innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK2 - Observation of Heterokaryons Through Cell Fusion

Kit 2: Observation of Heterokaryons Through Cell Fusion

Cell fusion has played an important role in gene mapping and hybridoma formation. This kit contains cells of two different species (mouse L929 cells and human fibroblasts) which have been fused and fixed to coverslips prior to shipping. Following a staining and mounting procedure, each student will observe the fused cells and tabulate the incidence of successful heterokaryon formation (mouse cell with human cell).

The manual discusses the methods by which cells are fused and the practical applications of this technology by biomedical researchers. This kit provides a unique way of introducing somatic cell genetics and hybridoma technology and their applications.

Cell fusion has played an important role in gene mapping and hybridoma formation. This kit contains cells of two different species (mouse L929 cells and human fibroblasts) which have been fused and fixed to coverslips prior to shipping. Following a staining and mounting procedure, each student will observe the fused cells and tabulate the incidence of successful heterokaryon formation (mouse cell with human cell).

The manual discusses the methods by which cells are fused and the practical applications of this technology by biomedical researchers. This kit provides a unique way of introducing somatic cell genetics and hybridoma technology and their applications.

IMR-90 mormal diploid human fibroblast cell line
Suitable for introductory biology, genetics, and immunology courses.

$65.00 Each Kit includes:

  • Enough materials for 30 students working individually
  • IMR-90 cells, a normal diploid human fibroblast cell line fixed to coverslips
  • L929, a transformed mouse cell line, fixed to coverslips.
  • STAIN #1 and STAIN #2.
  • Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, and a Further Reading Section.

Teacher's Preparation

  1. Monocular or binocular microscope (10 - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

60-80 minutes, including casual observations and calculations. There is a logical stopping point for those who are not on block schedule.

Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK3 - Effects of Cytotoxins on Cells

Kit 3: Effects of Toxic Chemicals on Cells

With this kit, each student will assess the various types and levels of abnormalities which may arise in cells following exposure to a cytotoxin. Mouse L929 cells are grown on coverslips in the presence of both low and high levels of colchicine. Each student will stain and mount the cells and observe with a microscope the effects of the toxin on cell morphology. Students compare these aberrant morphologies with those of untreated cells.

This exercise provides a practical introduction to the field of in vitro toxicology and its importance in biomedical research and environmental science.

The manual provides insights into the practical applications of this important field of study. The accompanying photographs illustrate some typical examples of cellular aberrations.

Suitable for introductory biology, cell biology, cytopathology, and environmental science courses.

$65.00 Each Kit includes:

  • Enough materials for 30 students working individually
  • L929 cells, a transformed mouse cell line, fixed to coverslips: zero dose.
  • L929 cells exposed to 1µg/ml of colchicine and fixed to coverslips
  • L929 cells exposed to 10µg/ml of colchicine and fixed to coverslips
  • STAIN #1 and STAIN #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Monocular or binocular microscope (10X - 40X objectives)
  2. Forceps, pipettes, microscope slides.
  3. Distilled or deionized water aliquotted in small tubes or in squeeze bottles

Time Requirements

60-80 minutes, including casual observations and calculations.

CSK4 - Preparation of Human Chromosome Spreads

Kit 4: Preparation of Human Chromosome Spreads

Using this kit, each student will be provided the unique opportunity of preparing a chromosome spread using human cells. The excitement generated by the students as they "splat" the cells onto microscope slides and proceed to "hunt" for the ideal chromosome spread with the microscope has generated an equal level of excitement among the hundreds of teachers who use this kit. Because the cells used for this exercise are transformed, the number of chromosomes will vary among the cells. Each student can count the chromosomes and see firsthand the range of aneuploidy.

The manual provides information regarding chromosome structure and the important role of karyotyping in geneticanalysis and genetic disease diagnosis.

Suitable for introductory biology, genetics, cytogenetics, cell biology, and oncology courses.

$65.00 Each Kit includes:

  • Enough materials for 30 students working individually.
  • 15 tubes each containing metaphase-blocked cell suspension, fixed in acetic acid-methanol fixative.
  • Stain #1 and Stain #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion) will enable the students to identify and count chromosomes ans see sister chromatids.
  2. Pipettes, microscope slides, coverslips.
  3. Staining jars or beakers ( = 250ml) containing room temperature water.

Time Requirements

40-60 minutes.

Innovative, inexpensive self-contained kits and PowerPoint presentations for unique hands-on laboratory experiences and instruction in biology, biotechnology and genetics.

CSK5 - Mouse Chromosome Studies

Kit 5: Mouse Chromosome Studies

Familiarity with mouse chromosomes is warranted because of the pivotal role of the mouse as an experimental organism and its importance in comparative karyotyping which serves to assist in the identification and mapping of human genes.

Kit 5, Mouse Chromosome Studies, is designed to introduce students to the basic principles entailed in the manipulation of mammalian cells so that chromosomes may be readily visualized, identified and studied. As mouse cells are cultured, their karyotype becomes varied due to an accumulation of translocations and other structural aberrations. Hence the cells in this kit provide a unique opportunity for the students to identify different structural aberrations and try to explain their origins.

Fixed swollen cells arrested in metaphase are provided for student use. The student will affix the cells to microscope slides and stain. These slides are scanned for good chromosome spreads which are observed at high magnification. Students search for normal and aberrant chromosomes, identifying the position of the centromere and relative sizes of chromosome arms.

The manual provided will describe how the cells were prepared for karyotyping, and subsequent steps exercised by the students. It also describes chromosome structure and structural aberrations.

Suitable for introductory biology, genetics, cytogenetics, cell biology, and oncology courses.

$65.00 Each Kit includes:
  • Enough materials for 30 students working individually.
  • 15 tubes each containing metaphase-blocked mouse cell suspension, fixed in acetic acid-methanol fixative.
  • Stain #1 and Stain #2; Permount (mounting medium).
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  1. Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion) will enable the students to identify and count chromosomes ans see sister chromatids.
  2. Pipettes, microscope slides, coverslips.
  3. Staining jars or beakers ( = 250ml) containing room temperature water.

Time Requirements

40-60 minutes.

CSK6 - Chromosome Banding

Kit 6: Chromosome Banding

Kit 6 consists of a set of five G-banded chromosome slides and one PowerPoint presentation describing chromosome banding, methods and applications. The slides provide an opportunity to examine chromosomes banded by the widely used G-banding technique. Hence, the descriptive presentation of the PowerPoint can be enriched by a hands-on microscopy exercise. The cells used as a source of chromosomes is the human cancer cell line, HeLa.

Topics: Definition and Importance of Chromosome Banding in Basic and Clinical Diagnosis; The Ideal Karyotype; From Cell Cultures or Biopsies to Chromosome Spreads for Karyotyping; Solid Stained and Banded Chromosomes Compared; Selected Aspects of Chromosome Nomenclature; Role of Banding in Chromosome Identification; Preparation of Chromosomes for Banding: Avoidance of Condensation, Aged Spreads, Baked Spreads; Trypsin Treatment; Q (Quinicrine), G (Giemsa), R (Reverse), and C (Centromere) Banding: Methods and Outcome; The Paris Conference Standard; Base Composition and Bands; Relaxation to Increase Resolution and Band number; Comparison of Banding Patterns of 350, 800, and 1200 Band Preparations; Analysis of Chromosome Aberrations Including Deletions, Additions, and Translocations; Other Banding Techniques and their Applications.

Also suitable for genetics, cytogenetics, cell biology, and oncology courses.

$99.00 Each Kit (includes companion PowerPoint Presentation)

Because banding requires diligent oversight and care as well as standardized trypsin treatment, we provide for class use stained slides of chromosomes. Instructors can also purchase baked slides of cells which require trypsin-treatment and Giemsa staining. We recommend unstained/untreated chromosome slides be purchased only for small groups of careful students.

5 additional G-Banded chromosome slides - $60
5 unstained baked slides, but not trypsin treated cells - $50

Kit includes:

  • 5 G-banded chromosome slides
  • Chromosome Banding PowerPoint Presentation (CSPP8)
  • PowerPoint Instructor's Guide
  • Printed background and procedural information as well as a Glossary of Terms, References, & Further Reading.

Teacher's Preparation

  • Binocular or monocular microscopes - magnification of 400X to 1000X (oil immersion).

Time Requirements

40-60 minutes.

Powerpoints

CSPP1 Normal and Cancer Cells In Vitro

PowerPoint Presentation Topics:

  • The Importance of in vitro Technology: Cell, Tissue and Organ Culture Defined
  • Use for Basic and Medical Research: Gene Mapping, Genetic Disease, Growth Factors, The Cell Cycle, Carcinogenesis
  • From Biopsy to Culture: Importance of Sterility, Biopsy vs. Whole Animal Embryo as a Cell Source, Tissue Mincing, Tissue Dissociation with Proteolytic Enzymes, The Primary Culture - Survival, Proliferation, and Elimination
  • The In Vitro Environment: Physical Environment & Chemical Environment
  • Normal Cells: Formation, Attachment, Contact Inhibition, Finite Life Span, and Senescence
  • Cancer Cells: In Vivo Behavior, In Vitro Properties, Evaluation of Tumorigenic Potential, Immortal Cells, Chromosome Abnormalities.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP2 Polymerase Chain Reaction (PCR)

PowerPoint Presentation Topics:

  • The Polymerase Chain Reaction (PCR) Defined
  • The Birth of PCR
  • Molecular Events of the Cycle: Denaturation, Primer Annealing, Primer Extension
  • Final Extension
  • Oligonucleotide Selection: Primer Design and Usage
  • Primer Length and Melting Temperature (Tm)
  • Base Composition
  • Using BLAST for Assessing Primer Design
  • Using the Internet and Software Programs for Primer Design and Usage
  • Properties of Thermostable DNA Polymerases
  • Optimization of the Reaction: A Typical Reaction Mix, Importance of Mg2+ Concentration, Guidelines for Determining Number of Cycles, PCR Additives for Optimization
  • PCR Contamination: Prevention and Control: Variations on the PCR Theme.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP3 Principles of Gel Electrophoresis

PowerPoint Presentation Topics:

  • General Considerations and Principles: The Electrophoretic Matrix: Properties of Agarose and Acrylamide
  • An Introduction to the theory of Electrophoresis: Interrelationship of Resistance, Voltage, Current and Power, Electrolysis and pH Effects
  • Nucleic Acid vs. Protein Separation
  • Denaturing vs. Non-Denaturing (Native) Electrophoresis
  • Sample Preparation; Comparison of Buffer Systems for Nucleic Acid Separations
  • Comparison of Buffer Systems for Protein Separation
  • Detection of Nucleic Acids in Agarose and Acrylamide Gels
  • Detection of Nucleic Acids on Blot Transfer Membranes
  • Detection of Proteins in Acrylamide Gels
  • Detection of Proteins on Blot Transfer Membranes.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP4  Karyotyping

PowerPoint Presentation Topics:

  • Karyotyping Defined
  • Representative Applications in Biomedical Studies
  • Chromosome Structure and Changes During the Cell Cycle
  • The Human Karyotype: Homologous Chromosomes, Somatic & Sex Chromosomes, and Chromosome Nomenclature
  • Manipulation of Cultured Cells for Karyotyping
  • Banded Chromosomes
  • Human Genome Project and Mapping of Disease and Normal Genes

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP5 Cell Fusion

A Powerful Tool for Monoclonal Antibody Production, Somatic Cell Genetics and Gene Mapping to Chromosomes

PowerPoint Presentation Topics:

  • Cell Fusion-- from Cytopathology Observation to Controlled Experimentation
  • The Age of Manipulative Biology and the Deliberate Manufacture of Somatic Cell Intra-species and Inter-species Hybrids
  • Overview: Fusion Partners (Parental Cells), The Fusion Process from Heterokaryons to Hybrids, The HAT Selection System
  • Complementary Parental Cells for TK and HGPRT genes
  • Hybrid Cell Line Stability and Random Loss of Genes
  • Alternative Fusion Methods
  • Use of Hybrids in Cancer Research: Fusion of Normal and Cancer Cells
  • Fusion of Antibody Producing Cells and Tumor Cells to Obtain an Immortal Hybridoma Cell Line Secreting One Antibody (Monoclonal Antibody Production)
  • Hybrid Cell Lines and Gene Mapping: Fusion Partners, Clonal Lines with Varying Karyotype, Correlation of Loss of Biochemical Trait with Loss of Specific Chromosome
  • Cell Reconstruction: The Assembly of Functional Cells with Organelles from Different Cells

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP6 Toxicology and Toxicity Testing

PowerPoint Presentation Topics:

  • Toxicology as a Science: Basic Research Goals and Application to Environmental Safety
  • The Imperative and Mandate for Chemical Testing
  • Toxicology Strategies: Dose and End-point for Toxicity
  • Whole Animal Models: Draize Test, LC50 Assay, Carcinogenesis, Developmental Toxicity
  • In Vitro Toxicology as a Complement to Animal Studies: Is it an Alternative?
  • Common in vitro Tests: Genotoxicity (Mutangenesis), Cell Transformation/ Carcinogenesis, Chorioallantoic Membrane Assay, Cytotoxicity Assays
  • Biotransformation- Metabolic Activation: Need for Supplementation
  • In Vitro-In Vivo Test Systems
  • Use of In Vitro Test Data in Decision Making

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP7 Immunology

The presentation introduces the cellular players and molecules involved in immune responses against pathogens, tumors, allergens or self-antigens.

PowerPoint Presentation Topics:

  • The Origin and Function of Cells of the Immune System
  • Functional Subsets of Immune Cells
  • In-Depth Discussion of Adaptive Versus Innate Immunity
  • Antigen Processing and Presentation
  • Structure and Function of Antigen Receptors on Lymphocytes
  • Antigen Recognition
  • Initiation and Control of the Immune Response
  • Soluble Factors of the Immune System

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP8 Chromosome Banding

PowerPoint Presentation Topics:

  • Definition and Importance of Chromosome Banding in Basic and Clinical Diagnosis
  • The Ideal Karyotype
  • From Cell Cultures or Biopsies to Chromosome Spreads for Karyotyping
  • Solid Stained and Banded Chromosomes Compared
  • Selected Aspects of Chromosome Nomenclature
  • Role of Banding in Chromosome Identification
  • Preparation of Chromosomes for Banding: Avoidance of Condensation, Aged Spreads, Baked Spreads
  • Trypsin Treatment
  • Q (Quinicrine), G (Giemsa), R (Reverse), and C (Centromere) Banding: Methods and Outcome
  • The Paris Conference Standard
  • Base Composition and Bands
  • Relaxation to Increase Resolution and Band Number
  • Comparison of Banding Patterns of 350, 800, and 1200 Band Preparations
  • Analysis of Chromosome Aberrations Including Deletions, Additions, and Translocations
  • Other Banding Techniques and their Applications.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each

CSPP9 Immunocytochemistry

PowerPoint Presentation Topics:

  • Cytochemistry and Immunocytochemistry Compared
  • Exploitation of the Affinity and Exquisite Specificity of the Antibody-Antigen Reaction
  • The Antibody-Antigen Reaction
  • Direct and Indirect Immunocytochemical Staining Approaches: Methods and Attributes
  • Identification and Localization of Antigens Within and on the Surface of Cells
  • Tags or Labels Conjugated to Antibodies Serve as Reporters Which Reveal, With the Aid of Appropriate Microscopes, the Site of the Antigen
  • Enzymatic Reporters and Their Substrates
  • Fluorescent Tags: Excitation and Fluorescence
  • Fluorescent Microscopes and Filters
  • Cells and Tissue Preparation: Fixation, Reduction of Background Caused by Cellular Chemicals, Permeabilization, and Attachment of Cells to Slides
  • Properties of Fluorescent Probes and their Selection
  • Non-Toxic Probes for the Study of Behavior of Live Cells
  • Representative Protocols.

PowerPoint Presentations: Each 35 - 45 frame presentation contains text in an outline form, charts, graphs and copies of microscopic observations. The material is supported by a manual for the instructor to follow, giving him or her additional information for each frame of the presentation. The presentations are designed to be covered in a 35 - 40 minute class period.

Price: $39.00 Each